2024

Pumroy, R. A., De Jesus-Perez, J. J., Protopopova, A. D., Rocereta, J. A., Fluck, E. C., Fricke, T., Lee, B. H., Rohacs, T., Leffler, A., and Moiseenkova-Bell, V. (2024) Molecular details of ruthenium red pore block in TRPV channels EMBO Reports  ahead of print  PMID:38225355

De Jesus-Perez, J. J., Gabrielle, M., Raheem, S., Fluck, E. C., Rohacs, T., and Moiseenkova-Bell, V. Y. (2024) Structural mechanism of TRPV5 inhibition by econazole Structure 32, 148-156 e145  PMID: 38141613

2023

Rohacs, T.: Phosphoinositide Regulation of TRP Channels: A Functional Overview in the Structural Era Annual  Review of  Physiology  (2023) ahead of print PMID: 37871124

Lee, B.H.; De Jesus Perez, J.J.; Moiseenkova-Bell, V.; Rohacs, T. Structural basis of the activation of TRPV5 channels by long-chain acyl-Coenzyme-A, Nature Communications, 14(1):5883 (2023)  PMID: 37735536

Gabrielle, M, Rohacs, T, TMEM120A/TACAN: A putative regulation or ion channels, mechanosensation and lipid metabolism, Channels, 17(1):2237306 PMID: 37523628

Burglen, L., Van Hoeymissen, E., Qebibo, L., Barth, M., Belnap, N., Boschann, F., Depienne, C., De Clercq, K., Douglas, A. G. L., Fitzgerald, M. P., Foulds, N., Garel, C., Helbig, I., Held, K., Horn, D., Janssen, A., Kaindl, A. M., Narayanan, V., Prager, C., Rupin-Mas, M., Afenjar, A., Zhao, S., Ramaekers, V. T., Ruggiero, S. M., Thomas, S., Valence, S., Van Maldergem, L., Rohacs, T., Rodriguez, D., Dyment, D., Voets, T. & Vriens, J. Gain-of-function variants in the ion channel gene TRPM3 underlie a spectrum of neurodevelopmental disorders. Elife 12, e81032, (2023) PMID: 36648066

2022

Zhao, S., Carnevale, V., Gabrielle, M., Gianti, E. & Rohacs, T. Computational and functional studies of the PI(4,5)P₂ binding site of the TRPM3 ion channel reveal interactions with other regulators. J Biol Chem, 298(11): 102547, (2022). PMID: 36181791

Rohacs, T., Fluck, E. C., De Jesus-Perez, J. J. & Moiseenkova-Bell, V. Y. What structures did, and did not, reveal about the function of the epithelial Ca²⁺ channels TRPV5 and TRPV6. Cell Calcium 106, 102620, (2022). PMID: 35834842

Fluck, E. C., Yazici, A. T., Rohacs, T. & Moiseenkova-Bell, V. Y. Structural basis of TRPV5 regulation by physiological and pathophysiological modulators. Cell Rep 39, 110737, (2022). PMID: 35476976

Del Rosario, J. S., Gabrielle, M., Yudin, Y. & Rohacs, T. TMEM120A/TACAN inhibits mechanically activated PIEZO2 channels. J Gen Physiol 154, (2022). PMID: 35819364

Balzer, M. S., Rohacs, T. & Susztak, K. How Many Cell Types Are in the Kidney and What Do They Do? Annu Rev Physiol 84, 507-531, (2022). PMID:34843404

2021

Yudin, Y; Liu, L; Nagwekar, J; Rohacs, T. (2021) Methods to study phosphoinositide regulation of ion channels, Methods in Enzymology, 2021; 652:49-79. PMID:34059290

Su, S., Yudin, Y., Kim, N., Tao, Y. X., and Rohacs, T . (2021) TRPM3 Channels Play Roles in Heat Hypersensitivity and Spontaneous Pain after Nerve Injury. J Neurosci 41, 2457-2474, PMID: 33478988

Jiang, W., Del Rosario, J. S., Botello-Smith, W., Zhao, S., Lin, Y. C., Zhang, H., Lacroix, J., Rohacs, T., and Luo, Y. L. (2021) Crowding-induced opening of the mechanosensitive Piezo1 channel in silico. Commun Biol 4, 84, PMID: 33469156

Kelemen, B., Pinto, S., Kim, N., Lisztes, E., Hanyicska, M., Vladar, A., Olah, A., Penzes, Z., Shu, B., Vriens, J., Biro, T., Rohacs, T., Voets, T., and Toth, B. I. (2021) The TRPM3 ion channel mediates nociception but not itch evoked by endogenous pruritogenic mediators. Biochem Pharmacol 183, 114310, PMID: 33130130

2020

Liu, L., Rohacs, T. (2020) Regulation of the cold-sensing TRPM8 channels by phosphoinositides and G _q-coupled receptors. Channels 14(1):79-86. PMID: 32101066

2019

Hughes, T. E.*, Del Rosario, J. S.*, Kapoor, A.*, Yazici, A. T., Yudin, Y., Fluck, E. C., Filizola, M.^#, Rohacs, T.^#, and Moiseenkova-Bell, V. Y.^# (2019) Structure-based characterization of novel TRPV5 inhibitors. Elife (2019) 8 pii: e49572 PMID: 31647410

Pumroy, R. A., Samanta, A., Liu, Y., Hughes, T. E., Zhao, S., Yudin, Y., Rohacs, T., Han, S. & Moiseenkova-Bell, V. Y. Molecular mechanism of TRPV2 channel modulation by cannabidiol. Elife, (2019) 8. pii: e48792. PMID:31566564

Luyu Liu, Yevgen Yudin, Janhavi Nagwekar, Chifei Kang, Natalia Shirokova, Tibor Rohacs, Gαq sensitizes TRPM8 to inhibition by PI(4,5)P₂ depletion upon receptor activation, J. Neurosci (2019) 39(31):6067-6080, PMID:31127000

Yudin Y, Rohacs T. The G protein-biased agents PZM21 and TRV130 are partialagonists of μ-opioid receptor-mediated signaling to ion channels. Br J Pharmacol. (2019) 176(17):3110-3125, PMID:31074038

Robinson, C.V., Rohacs, T., Hansen S.B.: Tools for Understanding Nanoscale Lipid Regulation of Ion Channels. Trends Biochem Sci. (2019) 44(9):795-806, PMID: 31060927

2018

Kasimova, M.A.,  Yazici, A.Y., Yudin, Y., Granata, D.,  Klein, M.L., Rohacs, T.,  Carnevale, V.: A hypothetical molecular mechanism for TRPV1 activation that invokes rotation of an S6 asparagine, Journal of General Physiology (2018) 50(11):1554-1566   PMID:30333107

Hughes, T.E.T.*,  Pumroy, R.A.*, Yazici, A.T.*, Kasimova, M.A.,  Fluck, E.C.,  Huynh, K.W.,  Samanta, A., Mulugu, S.K.,  Zhou, Z.H., Carnevale, V.,  Rohacs,T., Moiseenkova-Bell,V.Y.: Structural insights on TRPV5 gating by endogenous modulators, Nature Communications, (2018) 9(1):4198 PMID:30305626

Yudin, Y., Rohacs, T. Signaling by Gi/o-coupled receptors in somatosensory neurons; potential targets for novel pain medications, Molecular Pain (2018) 14:1-16 PMID: 29580154

Kasimova, M., Yazici, A., Yudin, Y., Granata, D., Klein, M., Rohacs, T., and Carnevale, V. (2018). Ion Channel Sensing: Are Fluctuations the Crux of the Matter? The Journal of Physical Chemistry Letters 9, 1260-1264. PMID: 29439562

Hughes, T.E.T., Lodowski, D.T., Huynh, K.W., Yazici, A., Del Rosario, J., Kapoor, A., Basak, S., Samanta, A., Han, X., Chakrapani, S., Zhou, Z.H., Filizola, M., Rohacs, T., Han, S., and Moiseenkova-Bell, V.Y. (2018). Structural basis of TRPV5 channel inhibition by econazole revealed by cryo-EM. Nat Struct Mol Biol 25, 53-60. PMID: 29323279

2017

Badheka, D.*, Yudin, Y.*, Borbiro, I., Hartle, C. M., Yazici, A., Mirshahi, T. & Rohacs, T. Inhibition of Transient Receptor Potential Melastatin 3 ion channel by G-protein βγ  subunits. eLife (2017) 6. pii: e26147. PMID: 28829742

Beckerman, P., Bi-Karchin, J., Park, A. S., Qiu, C., Dummer, P. D., Soomro, I., Boustany-Kari, C. M., Pullen, S. S., Miner, J. H., Hu, C. A., Rohacs, T.,Inoue, K., Ishibe, S., Saleem, M. A., Palmer, M. B., Cuervo, A. M., Kopp, J. B. & Susztak, K. Transgenic expression of human APOL1 risk variants in podocytes induces kidney disease in mice. Nature Medicine 23, 429-438, (2017). PMID: 28218918

Borbiro, I; Rohacs, T.Regulation of mechanosensitive Piezo channels by second messenger pathways, Curr Top Membr (2017) 79, 245-261, PMID: 28728819

2016

Carnevale, V. & Rohacs, T. TRPV1: A Target for Rational Drug Design. Pharmaceuticals (Basel) 9, (2016). PMID: 27563913

Velisetty, P.; Borbiro I., Kasimova, M.A.; Liu, L.; Carnevale, V.; Rohacs, T.A molecular determinant of phosphoinositide affinity in mammalian TRPV channels, Sci Rep. 2016, 6:27652 PMID: 27291418

Yudin, Y.; Rohacs, T.Phospholipase C δ4 regulates cold sensitivity in mice J. Physiol. 2016, 594(13):3609-28 PMID: 27062607

Elokely KM, Velisetty P, Delemotte L, Palovcak E, Klein ML, Rohacs Tand Carnevale V.Understanding TRPV1 Activation by Ligands: Insights from the Binding Modes of Capsaicin and Resiniferatoxin Proc Natl Acad Sci USA: 2016, 113(2):E137-45 PMID: 26719417

Rohacs, T. Phosphoinositide signaling in somatosensory neurons, Adv Biol Reg, 2016 61:2-16 PMID: 26724974

2015

Badheka D, Rohacs T. TRPM3 joins the ranks of PI(4,5)P₂sensitive ion channels. Channels 2015; 9(5): 233-4.

Badheka, D, Borbiro I, Rohacs, T. Transient receptor potential melastatin 3 is a phosphoinositide-dependent ion channel. J Gen Physiol. 2015 146(1):65-77

Rohacs T. Phosphoinositide regulation of TRPV1 revisited. Pflugers Arch.2015; 467 (9):1851-69

Borbiro, I, Badheka, D. Rohacs, T. Activation of TRPV1 channels inhibits mechanosensitive Piezo channel activity by depleting membrane phosphoinositides. Science Signaling, 2015; 8(363):ra15

2014

Rohacs, T., Phosphoinositide regulation of TRP channels, Handb Exp Pharmacol, 2014;223:1143-76

2013

Lukacs, V*., Rives, J.M.*, Sun, X., Zakharian, E., Rohacs, T.Promiscuous activation of transient receptor potential vanilloid 1 channels by negatively charged intracellular lipids, the key role of endogenous phosphoinositides in maintaining channel activity, J. Biol. Chem. 2013 288(49):35003-13

Rohacs T., Regulation of transient receptor potential channels by the phospholipase C pathway. Adv Biol Regul. 2013 53(3):341-55

Cao C, Yudin Y, Bikard Y, Chen W, Liu T, Li H, Jendrossek D, Cohen A, Pavlov E, Rohacs T, Zakharian E. Polyester Modification of the Mammalian TRPM8 Channel Protein: Implications for Structure and Function. Cell Reports.2013 4(2):302-15

Lukacs V, Yudin Y, Hammond GR, Sharma E, Fukami K, Rohacs T. Distinctive Changes in Plasma Membrane Phosphoinositides Underlie Differential Regulation of TRPV1 in Nociceptive Neurons. J Neurosci. 2013 33(28):11451-63

Cao C, Zakharian E, Borbiro I, Rohacs T. Interplay between calmodulin and phosphatidylinositol 4,5-bisphosphate in Ca²⁺-induced inactivation of transient receptor potential vanilloid 6 channels. J Biol Chem.2013; 288(8):5278-90.

Rohacs T, Recording macroscopic currents in large patches from Xenopus oocytes, Methods Mol Biol. 2013; 998:119-31

Rohacs, T. Sphingosine and the Receptor Potential Channel Kinase(s), British Journal of Pharmacology, 2013; 168(6):1291-3

2012

Gamper N, Rohacs T. Phosphoinositide sensitivity of ion channels, a functional perspective. Subcell Biochem. 2012; 59:289-333

Rohacs T., Signal termination: how many different ways can you hit the brakes in biological systems Acta Physiol(Oxf). 2012, 204(4):465

Yudin, Y., Rohacs, T. Regulation of TRPM8 channel activity, Molecular and Cellular Endocrinology, 2012, 353(1-2):68-74

2011

Rohacs, T. Cool channel subunits reveal their independent interactions with menthol, invited perspective, Journal of Physiology, 2011, 589:4809

Yudin, Y., Lukacs V., Cao C., Rohacs, T. Hydrolysis of phosphatidylinositol 4,5-bisphosphate mediates desensitization of the cold sensor TRPM8 channels, Journal of Physiology, 2011, 589:6007-27.

Zakharian, E*., Cao, C*. Rohacs, T. Intracellular ATP supports TRPV6 activity via lipid kinases and the generation of PtdIns(4,5)P₂, FASEB J, 2011 25:3915-28.

2010

Eleonora Zakharian,Chike Cao,Tibor Rohacs, Gating of TRPM8 channels activated by cold and chemical agonists in planar lipid bilayers, J. Neurosci 2010, 30(37):12526-34

2009

Tibor Rohacs: Regulation of non-canonical transient receptor potential channels by phosphoinositides Cell Calcium 2009, 45: 554–565

Eleonora Zakharian, Baskaran Thyagarajan, Robert J. French, Evgeny Pavlov, and Tibor Rohacs: Inorganic polyphosphate modulates TRPM8 channels, PLoS ONE, 2009;4(4):e5404

Thyagarajan B, Benn BS, Christakos S, Rohacs T. Phospholipase C mediated regulation of TRPV6 channels: implications in active intestinal Ca²⁺transport. Mol. Pharmacol. 2009. 75(3):608-16

2008

T. Rohacs, B. Thyagarajan, V. Lukacs: Phospholipase C mediated modulation of TRPV1 channels, Molecular Neurobiology, 2008, 37(2-3):153-63

Thyagarajan, B., Lukacs, V., and Rohacs, T. Hydrolysis of phosphatidylinositol 4,5-bisphosphate mediates calcium induced inactivation of TRPV6 channels. J. Biol. Chem. 2008.283: 14980-14987

2007

Lopes CMB, Remon JI, Matavel A, Sui JL, Keselman I, Medei E, Shen Y, Rosenhouse-Dantsker A, Rohacs Tand Logothetis DE. Protein Kinase A Modulates PLC-Dependent Regulation and PIP₂ sensitivity of K⁺ Channels 1: 124-134, 2007.

T. Rohacs, Bernd Nilius: Regulation of Transient Receptor Potential (TRP) channels by phosphoinositides Pluggers Archive 455(1):157-68, 2007

Viktor Lukacs*, Baskaran Thyagarajan*, Peter Varnai, Andras Balla, Tamas Balla, Tibor Rohacs: Dual regulation of TRPV1 by phosphoinositides J Neurosci 27 (26):7070-80. 2007

 T. Rohacs: PIP₂ regulation of TRP channels Pluggers Archive 453(6):753-62, 2007

2006

P. Varnai, B. Thyagarajan, T. Rohacs and T. Balla: Rapidly inducible changes in phosphatidylinositol 4,5-bisphosphate levels influence multiple regulatory functions of the lipid in intact cells. J Cell Biol175: 377-382, 2006.

1998-2005

T. Rohács: Teaching Resource. TRP channels Science STKE 2005(282):tr14, 2005

T. Rohács#, C.M.B. Lopes, I. Michailidis, D.E Logothetis: PI(4,5)P₂regulates the activation and desensitization of TRPM8 channels through the TRP domain, Nature Neuroscience8:626-634, 2005

C.M.B. Lopes*, T. Rohács*, G. Czirjak, T. Balla, P. Enyedi, D. E. Logothetis: PIP₂hydrolysis underlies agonist-induced inhibition and regulates voltage gating of two-pore domain K⁺channels. J.Physiol. 564:117-29, 2005

X. Du, H. Zhang, C.M.B. Lopes, T. Mirshahi, T. Rohács, D.E. Logothetis: Characteristic interactions with PIP₂determine regulation of Kir channels by diverse modulators. J. Biol. Chem. 279:37271-81, 2004

M.B. Meyers, A. Fischer, Y.J. Sun, C.M.B. Lopes, Rohács, T. Y. Nakamura, Y.Y. Zhou, P.C. Lee, R.A. Altschuld, S.A. McCune, W.A. Coetzee, G.I. Fishman: Sorcin regulates excitation-contraction coupling in the heart Journal of Biological Chemistry278: 28865-28871, 2003

H. Zhang, L.C. Craciun, T. Mirshahi, T. Rohács, C.M.B. Lopes, D.E. Logothetis: PIP₂activates KCNQ channels and its hydrolysis underlies receptor mediated inhibition of M-currentNeuron37:963-75, 2003

T. Rohács, C.M.B. Lopes, T. Jin, P. Ramdya, Z. Molnár and D.E. Logothetis: Phosphoinositide specificity determines lipid regulation of Kir channel activity Proc. Natl. Acad. Sci. USA 100:745-50, 2003

Jin, L. Peng, T.Rohács, T.Mirshahi, K.W. Chan, R. Sanchez and D.E. Logothetis: The  subunits of G proteins gate a K⁺channel by pivoted bending of a transmembrane segment. Molecular Cell 10:469-81, 2002

C. M.B. Lopes, H. Zhang, T. Rohács, T. Jin, J.Yang and D. E. Logothetis:Alterations in Conserved Kir Channel-PIP₂ Interactions Underlie Channelopathies Neuron 34, 933–944, 2002

T. Rohács,C.M.B. Lopes, T. Mirshahi, T. Jin, H. Zhang and D. E. Logothetis: Assaying PIP₂ regulation of potassium channels. Methods in Enzymology 345:71-92, 2002

A. Spät, J. Pitter, Rohácsand G. Szabadkai: Stimulus-secretion coupling and mitochondrial metabolism in steroid-secreting cells. News Physiol Sci. 16:197-200, 2001

Spät A, Pitter J, Rohács T, Szabadkai G. Calcium signal and mitochondrial metabolism in steroid producing cells. Endocr Res. 2000; 26(4):615-6.

T. Rohács, J. Chen, G.D. Prestwich and D.E. Logothetis: Distinct specificities of inwardly rectifying K⁺channels for phosphoinositides Journal of Biological Chemistry 274, 36065-72, 1999

1990-1997

T. Rohács,K. Tory, A. Dobos, and A. Spät: Intracellular calcium release is more efficient than calcium influx in stimulating mitochondrial NAD(P)H formation in adrenal glomerulosa cells Biochemical Journal. 328: 525-28 1997

T. Rohács, G. Nagy and A. Spät: Cytoplasmic Ca²⁺signal and reduction of mitochondrial pyridine nucleotides in adrenal glomerulosa cells  Biochemical Journal 322: 785-92, 1997

Spät A, Rohács T, Horváth A, Szabadkai G, Enyedi P. The role of voltage-dependent calcium channels in angiotensin-stimulated glomerulosa cells. Endocr Res. 1996; 22(4):569-76.

Szabadkai, A. Horváth,T. Rohács, L. Vimláti, A. Spät and P. Enyedi: Expression of inositol 1,4,5-trisphosphate receptors in rat adrenocortical zones Journal of Steroid Biochemistry and Molecular Biology 57(1-2): 13-17, 1996

A. Spät, T. Rohács and L. Hunyady: Plasmalemmal dihydropyridine receptors modify the function of subplasmalemmal inositol 1,4,5-trisphosphate receptors: a hypothesis. Cell Calcium 15: 431-437, 1994

T. Rohács, A. Bagó, F. Deák, L. Hunyady, and A. Spät: Capacitative Ca²⁺ influx in adrenal glomerulosa cells. Possible role in angiotensin II response. Am. J. Physiol. 267: C1246-C1252, 1994

L. Hunyady, T. Rohács, A. Bagó, F. Deák, and A. Spät: Dihydropyridine-sensitive initial component of the ANG II-induced Ca²⁺response in rat adrenal glomerulosa cells. Am. J. Physiol. 266: C67-C72, 1994

A. Spät, I. Balla, T. Balla, P. Enyedi, G. Hajnóczky, and T. Rohács: Sustained stimulation of aldosterone production by angiotensin II is potentiated by nickel. Am. J. Physiol. 258: E555-E561, 1990

* contributed equally

# corresponding author